ScanLag: High-throughput Quantification of Colony Growth and Lag Time
نویسندگان
چکیده
منابع مشابه
ScanLag: High-throughput Quantification of Colony Growth and Lag Time
Growth dynamics are fundamental characteristics of microorganisms. Quantifying growth precisely is an important goal in microbiology. Growth dynamics are affected both by the doubling time of the microorganism and by any delay in growth upon transfer from one condition to another, the lag. The ScanLag method enables the characterization of these two independent properties at the level of coloni...
متن کاملHigh-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts
The quantification of bacteria in cell culture infection models is of paramount importance for the characterization of host-pathogen interactions and pathogenicity factors involved. The standard to enumerate bacteria in these assays is plating of a dilution series on solid agar and counting of the resulting colony forming units (CFU). In contrast, the virtual colony count (VCC) method is a high...
متن کاملPerformance of high-throughput DNA quantification methods
BACKGROUND The accuracy and precision of estimates of DNA concentration are critical factors for efficient use of DNA samples in high-throughput genotype and sequence analyses. We evaluated the performance of spectrophotometric (OD) DNA quantification, and compared it to two fluorometric quantification methods, the PicoGreen assay (PG), and a novel real-time quantitative genomic PCR assay (QG) ...
متن کاملHigh-throughput corrosion quantification in varied microenvironments
Harsh conditions ranging from those of biomedical implants to industrial oilfield applications prompt rapid assessment of whether and to what extent corrosion will occur. Here, we describe and demonstrate a novel high-throughput approach to quantify corrosion susceptibility based upon electrical resistance and colorimetric outputs. We illustrate this capability by quantifying aerobic corrosion ...
متن کاملAutomated Quantification of Colony Growth in Clonogenic Assays
The need to determine the behavior of cell colonies in cultures often arises in controlled biological experiments. The deployment of microassays involving multiple wells allows the assessment of parameters such as the stage of malignancy, growth constraints and chemical concentrations etc. The large amount of preprocessed data negates the use of manual counting methods and requires automatic im...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/51456